FM Minimal Medium Size:250g 3 g/L TRIS 1 M

3  g/L TRIS 1  M  121

1 CFU/ml Bacterial endotoxin < 0

3  mM  20  g/L EDTA Na2 20

2 at 25°C Suspend 18 gram powdered medium in 1 litre distilled water

FM Minimal Medium Size:250g 3 g/L TRIS 1 MThis media is based on well defined formulations of mineral salts, vitamins and amino acids. Two commonly used synthetic defined minimal Dictystelium Discoideum media are FM and SIH. FM medium developed by Franke and Kessin is used for transformation of Dictyostelium Discoideum and genomic studies. This formulation supports the growth of most strains that are capable of growing on HL5. SIH medium is a newly developed modification of FM with regards to