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Annealing/Extension: 60 °C 01:00 (fluorescence read) Detection chemistry / dyes Probe-based qPCR with FAM reporter dye (no quencher specified in kit instructions)
Total 10 µL Thermal cycling conditions Reverse transcription 50 °C 15:00 (1 cycle)
Annealing/Extension: 60 °C 01:00 (fluorescence read during this step) Instrument compatibility SHENTEK-96S
which serves as the core of the retromer complex
Sweepoviruses Probe qPCR Kit Chemicals (G-L) Annealing/Extension: 60 °C 01:00 (fluorescenceDescription Sweepoviruses Probe qPCR Kit is a PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe based real time PCR. Purified DNA is directly amplified using sequence specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real time monitoring of amplification. The resulting signals may be used for comparative analysis of target
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